Retno Arianingrum, Retno Sunarminingsih, Edy Meiyanto, Sofia Mubarika
para-Hydroxy meta methoxy chalcone (pHmMC), achalc one derivate of 3 -(4'-hydroxy-3'-methoxyphenyl)-1-phenyl-2-propene-1-on,was investigated on its cytotoxicity, apoptosis induction, and Bcl-2 expression against human breast cancer-T47D cell lines. The cytotoxic effect was analysed using MTT [3-(4,5 dimethyltiazol-2-yl)-2,5-diphenyltetrazoilium bromide] assay, the apoptotic effect was determined by Annexin-V flow cytometry method, and the expression of Bcl-2 was identified using immunocyto chemistry method. The research results showed that the pHmMC’s IC50 on T47D cells was 48 □ M, and it increased the apoptotic induction and decreased the Bcl-2 expression level. © 2016, International Journal of Pharmaceutical and Clinical Research. All rights reserved.
Department of Chemistry Education, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Yogyakarta, 55281, Indonesia; Faculty of Pharmaceutical, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
