Sri Atun, Retno Arianingrum
Kaempferia rotunda known as kunci pepet or kunir putih in Indonesia, has been traditionally used in as abdominal pain, sputum laxative, wounds, and diarrhea colic disorder. This study was conducted to determine anticancer activity of bioactive compounds from Kaempferia rotunda rhizome against human breast cancer in vitro and in vivo. The isolation of bioactive compounds from methanol extract K. rotunda was carried out by chromatographic method, and the structure was elucidated based on spectroscophy method. The in vitro cytotoxicity test was done on human breast cancer T47D cell lines by MTT ([3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. In vivo activity assay was done by xenografting female Balb C mice with human breast cancer T47D cell implantation into the mamae fat-pad. In vitro cytotoxicity assay against human breast cancer of chloroform extract of K. rotunda and pinostrobin (1) showing their IC50 were 41.72 µg/ mL and 59.8 µg/ mL respectively. In vivo assay of chloroform extract of K. rotunda and pinostrobin (1) by xenografting female Balb C mice showed the average of cancer incidence for each group is 50-100%. The growth of cancer volume from each groups appear on the fourth day, and reach a maximum cancer volume after the seventh day. The fastest cancer volume decrease occurred in group treatment with chloroform extract of K. rotunda in the dose of 500 mg/Kg bw, and group treatment with pinostrobin (1) in the dose of 20 mg/Kg bw. The bioactive compounds can repair breast tissue histopathology, and suppress c-Myc expression on mice with T47D breast cancer xenograft. These findings proved that K. rotunda rhizome is potential to be developed as breast cancer chemotherapeutic agent. © 2015, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.
Department of Chemistry Education, Yogyakarta State University, Jl. Colombo No. 1 Depok, Sleman, Yogyakarta, 55281, Indonesia
