Sri Atun, Sri Handayani, Melia Aliffiana, Hajar Nur Afifah, Anna Rakhmawati
The objectives of this research is to analyze antibacterial activity of nanoparticles produced by ethanol extract Boesenbergia rotunda loaded with chitosan and alginic acid againts Streptococcus mutans. Antimicrobial activity of the extract was screened for activities againts pathogenic bacteria S. mutans by the disk-diffusion method. The assay was done in triplicate, and chloramphenicol was used as the positive control. Different concentrations, 500 to 0.5µg/mL, of each sample showed dose-dependant antibacterial activity. The results presented in this study showed the maximum of diameter zone of inhibition from nanoparticles produced by ethanol extract B. rotunda loaded with chitosan and alginic acid at concentration of 500 µg/mL, while ethanol extract of B. rotunda at concentration of 50 µg/mL, and chloramphenicol at 250 µg/mL. The optimal incubation time on the diameter zone of inhibition against S. mutans of each sample was 6 hours. The effect of incubation time on the diameter zone of inhibition against S. mutants showed that nanoparticles produced by ethanol extract B. rotunda loaded with chitosan and alginic acid were relatively more stable than by ethanol extract of B. rotunda.The minimum inhibitory concentration of each sample against S. mutans was found to be 5 µg/mL. © 2017, International Journal of Drug Delivery Technology. All rights reserved.
Department of Chemistry Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, Indonesia; Department of Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Jl. Colombo No.1 Depok, Sleman, Yogyakarta, 55281, Indonesia
