Sri Atun, Retno Arianingrum
Objective: The main objectives of this research are to characterize the nanoparticles produced by chloroform fraction of Kaempferia rotunda loaded with alginic acid and combination of alginic acid-chitosan, and its biological activity test. Methods: Chloroform fraction of K. rotunda was loaded on alginic acid and combination of alginic acid-chitosan nanoparticles by ionic gelation method in various compositions. Characterizations of the products were investigated in particle size, zeta potential, and morphology by scanning electron microscopy (SEM). The biological activity of the products as an antioxidant was tested by the 2,2-diphenyl-1-picrylhydrazyl method. The cytotoxic effect was analyzed using 3-(4,5 dimethyltiazol-2-yl)-2,5-diphenyltetrazoilium bromide assay. Result: The alginic acid nanoparticles can be synthesized at the optimal mass ratio range of alginic acid:CaCl2 of 10:1 (% w/v), while the combination of alginic acid-chitosan nanoparticle at the optimal mass ratio range of alginic acid:chitosan of 10:1 (% w/v) and added calcium ion at 0.015% w/v. Antioxidant activity from alginic acid nanoparticle; combination of alginic acid-chitosan nanoparticle; and chloroform fraction from K. rotunda showed inhibition activity (IC50): 225.63±3.5, 237.12±2.1, and 25.20±1.3 mg/mL, respectively, whereas the cytotoxic effect against human breast cancer T47D from these compounds showed IC50: 145.25±2.2, 281.21±2.3, and 41.72±0.47 µg/mL, respectively. Conclusion: The nanoparticle products of chloroform fraction from K. rotunda loaded alginic acid and combination alginic acid-chitosan were successfully obtained by ionic gelation method. The nanoparticle products show lower activity in antioxidant and cytotoxic effects against human breast cancer T47D cell lines than the starting material chloroform fraction of K. rotunda. © 2017 The Authors.
Department of Chemistry Education, Faculty Mathematics and Natural Science, Yogyakarta State University, Sleman, Yogyakarta, Indonesia
