Andian Ari Anggraeni, Makiko Sakka, Tetsuya Kimura, Khanok Ratanakhaokchai, Motomitsu Kitaoka, Kazuo Sakka
A family-4 α-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD+, some divalent cations such as Mn2+, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans.
Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu 514-8507, 1577 Kurima-Machiyacho, Japan; School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand; National Food Research Institute, Tsukuba 305-8642, 2-1-12 Kannondai, Japan; Faculty of Engineering, State University of Yogyakarta, Yogyakarta 55281, Indonesia
