Afrizal Lathiful Fadli, Absari Hanifah, Ana Fitriani, Anna Rakhmawati, Wipsar Sunu Brams Dwandaru
Nosocomial infections in the hospital cause height level of morbidity and mortality. WHO research referring to nosocomial infections is approximately 8.7% of 55 hospitals from 14 countries in Europe, Arabian, and Asia. Staphylococcus aureus constitutes bacteria that cause nosocomial infections. Objectives of the research are to know how the step, characteristic, and activity of antibacterial for S.aureus from nanoparticle silver-chitosan, and the combination of both materials. The principle of nanosilver particle has antibacterial activity, was proved that silver ions are able to make strong inhibitions on bacteria cell wall. Silver ions will damage and penetrate cell wall bacteria then alternating of DNA bacteria. This condition makes fatality for bacteria. Chitosan was found by chitin deacetylation polyatomic with positive content and pH less than 6,5. Cationic of chitosan can make interaction with the cell wall of bacteria. Material combinations will make interactions and cause the damages on bacteria cell walls and can drag off the growing of bacteria cause of nosocomial infections. The result of the research indicated that silver nanoparticles with spectrums UV-Vis characterization, maximum absorption shows at wavelength 419 nm. The combination of silver nanoparticles and nanochitosan can drag the growth of bacteria S. aureus. The even more silver nanoparticle is working better than nano chitosan as antibacterial. As the conclusion, the application of Silver-Chitosan nanoparticles is effective to drag the growth of bacteria 0.62 cm in nutrient agar or medium of S. aureus that causes nosocomial infections. © 2018 Author(s).
Physics Department, Yogyakarta State University, Jl. Colombo No 1, Yogyakarta, Indonesia; Biology Department, Yogyakarta State University, Jl. Colombo No 1 Yogyakarta, Indonesia; Mathematics Department, Yogyakarta State University, Jl-Colombo-No-1, Indonesia
