M. Nugraheni, U. Santoso, Windarwati
Three different solvents were used to extract the phytochemical compounds in the peel and flesh of Coleus tuberosus. The results of extraction with different solvents were compared based on the levels of phytochemicals compounds and its antioxidant activity. Extraction of bioactive compounds was done by the maceration method using methanol, ethyl acetate, and chloroform for seven days at a ratio of 1:5 (w/v). Determination of total phenolic contents and flavonoids was conducted via spectrophotometric method. Antioxidant activity was evaluated based on DPPH, peroxide value and TBA value. The results showed that the methanol solvent extracted higher total phenolic and flavonoids compounds than the ethyl acetate and chloroform. The antioxidant activity of methanol extracts was higher compared to ethyl acetate and chloroform extracts for both flesh and peel of C. tuberosus. The extract of the peel of C. tuberosus had antioxidant activity higher compared to the flesh extract with the use of methanol, ethyl acetate or chloroform as solvent. There was a positive and significant correlation between total phenolic and flavonoids content against DPPH and a negative and significant correlation with peroxide and TBA value. © 2018 The Authors.
Department of Culinary Art Education, Engineering Faculty, Yogyakarta State University, Indonesia; Department of Food Science, Faculty of Agricultural Technology, Gadjah Mada University, Indonesia; Dr. Sardjito General Hospital, Health Ministry of Republic Indonesia, Yogyakarta, Indonesia
