Wipsar Sunu Brams Dwandaru, Afrizal Lathiful Fadli, Dani Rahmawati Hakim, Absari Hanifah, Emi Kurnia Sari
This study aims to i) synthesize silver nanoparticle-graphene oxide (Nano-Graver) mixture via liquid sonication exfoliation method using graphite from 2B pencil, ii) characterize the Nano-Graver solution based on UV-Vis, FTIR, XRD, and SEM, and iii) determine the Nano-Graver solution inhibition zone diameter against Staphylococcus aureus (S. aureus) bacteria. This is an experimental research with steps given as follows: I) synthesizing graphene oxide (GO) via the liquid sonication exfoliation (LSE) method, ii) synthesizing silver nanoparticles (AgNPs) chemically from AgNO3, iii) mixing the GO and AgNP solutions to become Nano-Graver, iv) characterizing the Nano-Graver solutions, and v) determining the Nano-Graver solution inhibition zone diameter against S. aureus bacteria via nutrient agar (NA) and blood agar (BA) media. The Nano-Graver solution is successfully synthesized having a dark-yellowish color, compared to a yellowish color solution of the AgNP and a clear solution of GO. The UV-Vis test of the Nano-Graver solution shows two absorbance peaks at wavelengths of 230 nm and 435 nm indicating the existence of GO and AgNP materials, respectively. The FTIR result shows OH and C = C groups, which means GO material occurs in the Nano-Graver solution. The XRD test shows that the Nano-Graver material has a semi-crystalline or amorphous structure. The SEM test for the Nano-Graver solution shows GO in coral-like material decorated by small spheres of AgNPs. The inhibition zone test of S. aureus bacteria produces the largest inhibition zone diameters in NA and BA media of 1.731 cm and 1.043 cm, respectively, for Nano-Graver concentration of 5 mM. © 2019 Author(s).
Department of Physics Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Jl. Colombo No. 1, Yogyakarta, 55281, Indonesia; Department of Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Jl. Colombo No. 1, Yogyakarta, 55281, Indonesia
