Qonitah Fardiyah, Taslim Ersam, Suyanta, Agus Slamet, Suprapto, Fredy Kurniawan
The species Andrographis paniculata L. Ness, family Acanthaceae grows in many parts in the island of Java in Indonesia. In this research, new potentials of photoprotective agents from A. paniculata plant extracts, along with their characteristics, were investigated. The phytochemical test, infrared analysis, ultraviolet characterization, fluorescence identification, flavonoid content by thin layer chromatography, high performance liquid chromatography-mass spectrometry (LCMS/MS) and photoprotective analysis were performed in the extracts. The new potentials of A. paniculata plant extracts were studied at various concentrations of extracts and shown by the value of the sun protection factor (SPF). Based on phytochemical test, A. paniculata plant extracts contained flavonoids, alkaloids, tannins, triterpenoids and polyphenols. A. paniculata plant extracts have 2 maximum wavelengths at 230 nm and 362 nm. The excitation peak at 300 nm (552.11 a.u) and the emission peak at 605 nm (516.02 a.u). The flavonoid content of the extracts was 0.022 µg/mL quercetin with Rf value of 0.61. The chromatogram of the LCMS/MS is similar to quercetin-3-glycoside standard at a retention time of 2.77. The photoprotective activity of A. paniculata plant extracts had ≥ 15 SPF. Its shows the possibility to use this extract as photoprotective agent in pharmaceutical preparations. © 2020 The Author(s)
Instrumentation and Analytical Sciences Laboratory, Department of Chemistry, Faculty of Sciences, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia; Analytical Chemistry Laboratory, Departement of Chemistry, Faculty of Mathematic and Natural Science, Brawijaya University, Malang, 65145, Indonesia; Natural Products and Synthesis Chemistry Research Laboratory, Department of Chemistry, Faculty of Sciences, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia; Analytical Chemistry Laboratory, Department of Chemistry Education, Faculty of Mathematics and Natural Science, Yogyakarta State University, Indonesia; Department of Environmental Engineering, Faculty of Civil, Planning, and Geo Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, 6011, Indonesia
