Anna Rakhmawati, Endang Tri Wahyuni, Triwibowo Yuwono
Lead uptake capacity of four lead-tolerant thermophilic bacteria Aeribacillus pallidus strains MRP112, MRP148, MRP272, and MRP278, originally isolated from Merapi volcano, were examined for their potential as an absorbent to uptake lead (Pb) from an aqueous solution. The growth profile and Pb uptake capacity were evaluated in Luria Bertani (LB) medium supplemented with Pb 100 mg/L. The optimum condition was evaluated under various analytical parameters, including temperature (55°C, 60°C, 65°C), pH (5, 6, 7), and contact time (6, 15, 24 h). The interaction of bacterial cell surface with ion Pb was proven using Fourier transform infrared spectroscopy (FT-IR). The optimal growth of all strains was observed at 55°C and pH 7. Aeribacillus pallidus MRP112 showed the highest uptake capacity of 97.41 ± 0.82 mg/g at 55°C, pH 5 for 15 h. FT-IR analysis displayed involved various functional groups such as hydroxyl, carboxyl, amine, and amide group ligands of protein in binding Pb ion. The A. pallidus strain represents a good candidate for the bioremediation of Pb-contaminated environment. © 2021. All rights reserved.
Biotechnology Study Program, Graduate School of Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Department of Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Yogyakarta, 55281, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia; Department of Agricultural Microbiology, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
