Ngadikun, Untung Widodo, Agus Barmawi, Ahmad Hamim Sadewa, Kuncoro Asih Nugroho, Metamalik Pasala
Colorectal cancer (CRC) is the third most common cancer in men and the second in women worldwide. To determine a biomarker for CRC, a new spectrophotometric method has been being developed. This study aims to investigate the pattern of EDTA-blood absorbance in CRC patients. One ml blood was collected from 52 subjects by venipuncture, which then drawn into EDTA tubes for spectrophotometry analysis. The differences of the parameters of EDTA-blood absorbance between two groups based on energetics of non-biological systems (ENBS) and energetics of biological systems (EBS) approach were tested by the t-test. The statistical significance of p<0.01 was used to evaluate the difference. The receiver operating characteristic (ROC) curves were applied to evaluate the effect of biomarkers on absorbance pattern. The t-test results indicate that the significant difference was observed between CRC patients and healthy controls only in parameter-1 of six parameters based on the ENBS approach. However, there were significant differences in all parameters based on the EBS approach. It was concluded that parameters of the pattern of EDTA-blood absorbance based on the EBS approach can be used to identify new biomarkers of CRC. © 2023 Author(s).
Department of Biochemistry, Faculty of Medicine Gadjah Mada University, Sleman District, Special Region, Yogyakarta, 55281, Indonesia; Department of Anesthesia and Intensive Care, Faculty of Medicine Gadjah Mada University, Sleman District, Special Region, Yogyakarta, 55281, Indonesia; Department of Surgery, Faculty of Medicine Gadjah Mada University, Sleman District, Special Region, Yogyakarta, 55281, Indonesia; Department of Physics Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Sleman District, Special Region, Yogyakarta, 55281, Indonesia
