Arief Priyadi, Paramita Cahyaningrum Kuswandi, Evy Yulianti, Risha Amilia Pratiwi, Ni Putu Sri Asih
In this study, the genome of an endemic Sulawesi's plant, Medinilla tapete-magicum was sequenced using Illumina NextSeq 500 and assembled the whole chloroplast genome. Results showed that the cpGenome is 155, 602 bp in size with typical quadripartite structure of a large single copy (LSC) region (85, 409 bp), a short single copy (SSC) region (16, 629 bp), and a pair of inverted repeats (IRs) regions (26, 782 bp). The cpGenome is composed of 132 genes, which consists of 87 protein coding genes, 37 tRNAs, and 8 rRNAs. The sliding window analyses showed that psbB-psbH and ndhF-rpl32 can potentially be used as markers. Microsatellite motifs of mononucleotide A and T dominated in the cpGenome. The phylogenetic trees from the concatenated 76 shared protein coding gene sequences showed the Medinilla clade was monophyletic and M. tapete-magicum is a sister species in the SE Asian clade which contain M. mag-nifica and M. speciosa. Copyright: © 2024, J. Tropical Biodiversity Biotechnology (CC BY-SA 4.0)
Research Center for Applied Botany, Research Organization for Life Sciences and Environment, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta - Bogor KM. 46 Cibinong, West Java, Bogor, 16911, Indonesia; Department of Biology Education, Faculty of Mathematics and Natural Sciences, Universitas Negeri Yogyakarta, Jl. Colombo Yogyakarta No.1, Karangmalang, Sleman, Yogyakarta, Depok, 55281, Indonesia; Research Center for Biosystematics and Evolution, Research Organisation for Life Sciences and Environment, National Research and Innovation Agency (BRIN), Jl. Raya Jakarta - Bogor KM. 46 Cibinong, West Java, Bogor, 16911, Indonesia
