Eli Rohaeti, Isana Supiah Yosephine Louise, Dini Rohmawati, Sekar Paramitha Rizky Diniati
The increasing waste of plastic is harmful to health and the environment so, biodegradable-based bio composite is made. This study aims to determine the antimicrobial activity characteristics of gelatin, chitosan, and glycerol composites with and without the addition of pandan leaf extract as bio composite materials. Bio composite was made from a composite of gelatin, chitosan, glycerol with a composition of 6:2:2.5 (v/v) which was added with 5 variations of pandan leaf extract concentration, namely 0%, 5%, 10%, 25%, 50% (v/v). Characterization was carried out including physical properties test, tensile testing, water solubility, FTIR test, SEM-EDX test, and antimicrobial activity test against Staphylococcus aureus, Escherichia coli, and Aspergillus sp. The characterization results showed that the addition of pandanus leaf extract had no effect on thickness, elongation, and water solubility, but had an effect on tensile strength, Young's modulus, and antimicrobial activity. The thickness test results show that the composite samples have a thickness of <0.25 mm in accordance with JIS standards. The results of the tensile strength, Young's modulus, and antimicrobial tests showed a significant difference between the composite samples with the best results in the composite sample with the addition of 25% pandan leaf extract. © 2025 Author(s).
Laboratory of Chemistry, Department of Chemistry Education, Faculty of Mathematics and Natural Sciences, Yogyakarta State University, Sleman, 55281, Indonesia
