Mudjijana, Nurvita Risdiana, Arya Adiningrat, Rela Adi Himarosa, Krisdiyanto, Rahmad Kuncoro Adi, Evy Yulianti, Fajar Sofyantoro, Muchamad Afif Ansori
The global pandemic announced by the World Health Organization (WHO) in January 2020 has infected many people in various countries. The Covid-19 pandemic, caused by SARS CoV-2, is a disease that is difficult to diagnose because of its non-specific clinical symptoms. Some observed clinical symptoms include fever, cough, or shortness of breath that occurs approximately two days after exposure to the virus. RT-qPCR is a method that can be used to detect the SARS CoV-2 virus, with high sensitivity and specificity. However, this method has several disadvantages, including requiring special equipment, a long process, high costs, and the need for special expertise from operators and analysts in implementing the method. RT-LAMP is an alternative method that can detect the SARS CoV-2 virus faster with high sensitivity. The advantages of RT-LAMP compared to the RT-qPCR method are that it requires less equipment, a relatively shorter time, and a lower cost to detect the SARS CoV-2 virus. Our current study reported that the RT-LAMP machine showed an accuracy of 96.67 % in detecting the presence of the SARS CoV-2 virus. © 2024 American Institute of Physics Inc.. All rights reserved.
Mechanical Engineering Department, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia; Faculty of Medicine and Health Science, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia; Department of Oral Biology and Biomedical Sciences, School of Dentistry, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia; Department of Mechanical and Industrial Engineering, Faculty of Engineering, Gadjah Mada University, Yogyakarta, Indonesia; Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta, Yogyakarta, Indonesia; Department of Animal Physiology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia